Comparing Defined- Substrate Coliform Tests for the Detection of Escherichia coli in Water
نویسندگان
چکیده
Two commercially available defined-substrate coliform tests were compared with EC medium plus 4-methylumbelliferyl-p-D-glucuronide (MUG) for detecting chlorine-exposed Escherichia coli in spiked water samples. Statistical analyses of the test results indicated no sign!nifcant differences in detection of E. cofi between the Autoanalysis Colilert test and EC medium with MUG. There were, however, significant differences in detection of E. coli between the ColiQuik test and EC medium with MUG in the free-chlorine-exposed pure culture studies and when all the data were combined. All methods were capable of detecting 1 cfu/lOO mL of E. cofi.
منابع مشابه
Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: comparison with defined substrate and plating methods for water quality monitoring.
Multiplex polymerase chain reaction (PCR) and gene probe detection of target lacZ and uidA genes were used to detect total coliform bacteria and Escherichia coli, respectively, for determining water quality. In tests of environmental water samples, the lacZ PCR method gave results statistically equivalent to those of the plate count and defined substrate methods accepted by the U.S. Environment...
متن کاملA comparison of the International Standards Organisation reference method for the detection of coliforms and Escherichia coli in water with a defined substrate procedure.
AIMS This study investigated the use of the International Standards Organisation (ISO) procedure for the comparison of microbiological methods. Using this procedure the ISO reference procedure for the detection of coliforms and Escherichia coli in water was compared with a defined substrate method (ColilertTM). METHODS AND RESULTS A total of 20 laboratories from 13 European countries compared...
متن کاملNational field evaluation of a defined substrate method for the simultaneous detection of total coliforms and Escherichia coli from drinking water: comparison with presence-absence techniques.
A defined substrate method was applied to drinking water to simultaneously enumerate total coliforms and total Escherichia coli directly from samples. After incubation at 35 degrees C for 24 h, the development of yellow in an initially colorless solution was specific for total coliforms; fluorescence at 366 nm in the same tube(s) or vessel demonstrated the presence of E. coli. No confirmatory o...
متن کاملThe Effect of Gamma (γ) Irradiation to inactivate Escherichia coli in Contaminated water
Several common approaches are used for disinfection of water in this section, but using the approaches (e.g. chlorination) are accompanied with hazardous by-products and also lead to notable changes in odor and flavor of water. Gamma irradiation as an emerging method has been encouraged currently by FAO/WHO for water, wastewater and food treatment. Coliform bacteria belong to common contaminant...
متن کاملEnumeration of total coliforms and Escherichia coli from source water by the defined substrate technology.
Many water utilities are required to monitor source water for the presence of total coliforms, fecal coliforms, or both. The Colilert system, an application of the defined substrate technology, simultaneously detects the presence of both total coliforms and Escherichia coli directly from a water sample. After incubation, the formula becomes yellow if total coliforms are present and fluorescent ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006